Introduction
Exposure to rotenone could directly affects the function of mitochondrial which leads to neurodegeneration. This study evaluated the therapeutic potential of taxifolin (Huāqí sōng dài; 花旗松甙) to alleviate metabolic and neurochemical alterations in the hippocampal and cortical region of brain of rotenone-toxified rats.
Methods
Male Wistar rats were subcutaneously administered 1.5 mg/kg rotenone for 10 days, followed by post-treatment with varying doses of taxifolin (0.25, 0.5 and 1.0 mg/kg) for 3 days through the same route. Activities of mitochondrial complex I, membrane ion pump and lactate dehydrogenase (LDH) were evaluated in the hippocampus and cortex of the brain of rotenone-toxified rats. Markers of neurotransmitter metabolism and oxidative stress were also biochemically estimated and molecular interaction between taxifolin and tyrosine hydroxylase, monoamine oxidase, glutamine synthetase and Na+/K+ATPase was determined by in silico simulation.
Results
Taxifolin attenuated dysfunction of mitochondrial, Na+/K+ATPase, LDH and modulate neurotransmitter metabolism. Also, the elicited oxidative stress was mitigated by taxifolin in the hippocampus and cortex of the brain of rotenone-toxified rats. Furthermore, taxifolin showed excellent interactions with targeted enzymes in silico. The highest binding affinity was recorded in taxifolin-tyrosine hydroxylase complex. Hydrogen bond and hydrophobic interactions were the two key molecular interaction between the taxifolin and targeted enzymes.
Discussion
Taxifolin alleviates metabolic and neurochemical aberrations in rotenone-induced neurotoxicity model in rats in vivo and exhibits promising interactions with specific target enzymes that are key to neurotransmission in silico.
Download the full paper: https://www.researchgate.net/publication/380446595_Taxifolin_Alleviate_Metabolic_and_Neurochemical_Alterations_in_the_Hippocampus_and_Cortex_of_Rats_with_Rotenone-Induced_Toxicity_In_Vivo_and_In_Silico_Insight
Or
https://www.sciencedirect.com/science/article/pii/S2667142524000824
Endorsed by: Heze-Sapience International
Heze-Sapience International 